Physicochemical and genetic evidence for specific antiestrogen binding sites.
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چکیده
منابع مشابه
Physicochemical and genetic evidence for specific antiestrogen binding sites.
In rat uterus and human breast cancer MCF-7 cell cytosol, the antiestrogens tamoxifen (Tam) and 4-hydroxytamoxifen (OH-Tam) bind to "antiestrogen binding sites" (ABS), which do not bind estradiol (E). Demonstrated in total cytosol by binding studies with radioactive antiestrogens in the presence of a large concentration of E, ABS can be physically separated from E-binding estrogen receptor (ER)...
متن کاملConformational and physicochemical DNA features specific for transcription factor binding sites
MOTIVATION A reliable recognition of transcription factor binding sites is essential for analysis of regulatory genomic sequences. The experimental data make evident an important role of DNA conformational features for site functioning. However, Internet-available tools for revealing conformational and physicochemical DNA features significant for the site functioning and subsequent use of these...
متن کاملInteractions of sterols with antiestrogen-binding sites: structural requirements for high-affinity binding.
Animal and human tissues contain a microsomal protein that binds nonsteroidal antiestrogens with high affinity and specificity. The functions of these binding sites and the identity of their natural ligands are unknown. Following a report that certain sterols inhibit [3H]tamoxifen binding to this site, we attempted to define the structural requirements for maximal inhibition of [3H]tamoxifen bi...
متن کاملCompetition of zinc, cadmium and calcium for binding sites in sperm of trout and carp
Heavy metal pollutions of aquatic ecosystems have already raised many concerns on aquaticorganisms’health and survival especially on their sperm. The effects of a single metal on sperm may be totallydifferent from cocktail of several metals because of their interactions, so the effects of zinc, cadmium andcalcium on the trout and carp sperms and their competition have been examined by incubatio...
متن کاملCharacterization and Quantitation of Antiestrogen Binding Sites in Estrogen Receptor-positive and -negative Human Breast Cancer
Antiestrogens are useful in the treatment of endocrine-respon sive breast cancers in humans. In an attempt to understand the mechanisms underlying their estrogen antagonism and antitumor character, we have examined the interaction of antiestrogens with three human breast cancer cell lines that differ markedly in their estrogen receptor content and in their sensitivity to growth suppression by a...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.11.3158